LYSOSOMAL FRACTIONS FROM TRANSITIONAL EPITHELIUM
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چکیده
منابع مشابه
Lysosomal Fractions from Transitional Epithelium
Histochemical data suggested that the so called lipoid granules of transitional epithelium in some species are equivalent to lysosomes. Scrapings of bovine and canine transitional epithelium were subjected to differential centrifugation to confirm this identification biochemically. Fractions of rat liver, the classic source of lysosomes, were also prepared by the same methods to compare with th...
متن کاملAcid ribonucleases of lysosomal and soluble fractions from rat liver.
1. Lysosomal acid RNase was partially purified from a rat liver homogenate and a lysosomal fraction. This RNase, of optimum pH 5 to 6, cleaved RNA to form nucleoside 2’,3’cyclic phosphates, which were hydrolyzed to nucleoside 3’-monophosphates. This enzyme was heat-labile and had a molecular weight of about 24,000 to 28,000, as estimated by gel filtration with Bio-Gel P-60. This lysosomal acid ...
متن کاملThe Permeability of Rat Transitional Epithelium
Permeability barriers must exist in transitional epithelium to prevent the free flow of water from underlying blood capillaries through the epithelium into the hypertonic urine, and such a barrier has now been demonstrated in isolated bladders. This barrier is passive in function and can be destroyed by damaging the luminal surface of the transitional epithelium with sodium hydroxide and 8 M ur...
متن کاملThe Fine Structure of the Transitional Epithelium of Rat Ureter
The fine structure of the transitional epithelium of rat ureter has been studied in thin sections with the electron microscope, including some stained cytochemically to show nucleoside triphosphatase activity. The epithelium is three to four cells deep with cuboidal or columnar basal cells, intermediate cells, and superficial squamous cells. The basal cells are attached by half desmosomes, or a...
متن کاملThe growth and differentiation of transitional epithelium in vitro
The development of rat transitional epithelial cells grown on conventional non-permeable surfaces was compared with development on permeable collagen supports. On glass or plastic surfaces, cells grew as expanding nomolayer sheets. Once confluent, growth continued with a bilayer being formed in most areas and apical cells being continuously sloughed off. Although most cells were interconnected ...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1965
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.24.2.259